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Optimal Recovery of Microbial DNA for 16S Sequencing from Murine Gastrointestinal Tract Tissue Through Bead Mill Homogenization and Automated Nucleic Acid Extraction
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Explore Cutting-edge Methods for Extracting Microbial DNA
- The microbiome, a complex network of microorganisms, plays a pivotal role in human health. Yet, accurately analyzing this ecosystem, especially from solid tissue samples, presents significant challenges. Overcoming biases in DNA extraction is crucial for authentic representation of microbial populations.
- In this Application Note, we explore an innovative approach combining bead mill homogenization with automated nucleic acid extraction. This method ensures thorough and efficient lysis of microbes, including those with robust cell walls, and offers an unbiased recovery of microbial DNA from GI tract tissue.
- By utilizing the OMNI Bead Ruptor Elite™ for mechanical lysis and the chemagic™ 360 for automated nucleic acid extraction, this protocol minimizes human error and maximizes reproducibility. It's a step forward in ensuring high-quality, consistent DNA extraction suitable for advanced applications like PCR and next-generation sequencing.
- Our methodology addresses the shortcomings of traditional sample analysis and paves the way for more accurate, repeatable, and scalable research in microbiome studies. Download our Application Note and discover how you can apply these advanced DNA extraction techniques in your lab.
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